After blocking in 5% skimmed milk powder for 2 hours at room temperature, the samples were incubated overnight at 4C with primary antibodies, including ERK1/2 (#9102), p-ERK1/2 (#9101), JNK1/2 (#9252), p-JNK (#9251), p38 MAPK (#9212), and p-p38 MAPK (#9211), which were obtained from Cell Signaling Technology (Beverly, MA, USA), as well as VEGF-a (sc-7269) and Tubulin (sc-8035), which were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA)
Take the injection on the same day each week and at the same time, as missing doses or inconsistent use may reduce the effectiveness of the medicine
The FDA's GRAS determination confirms safety for oral consumption as a food ingredient
In addition, the change of DNA methylation is rather different between early and late stage (294, 295)