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For glucose uptake and GLUT-1 surface detection splenocytes were washed with glucose-free RPMI medium (Lonza BE12-752F)
Disease activity was classified as remission, mild, moderate or severe based on rheumatology notes, and cardiometabolic measures and laboratory values were collected when available
And if they do, unfortunately, itll create a worse black market than there is now
Now the injection needle ( I use a 25G x 1inch ) is put on the syringe and any air left in the syringe is expelled until a tiny bead of B12 appears on the tip of the needle
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